Quantitative analysis of isorhamnetin-3-rutinoside in leaves of Nitraria Spp .from Xinjiang region / 药学实践杂志

ABSTRACT

Objective To provide supporting data for the quality control and evaluation of Nitraria Spp .from Xinjiang region by analyzing the content of isorhamnetin-3-rutinoside in its leaves .Methods HPLC method was used for the determina-tion of isorhamnetin-3-rutinoside in leaves of Nitraria Spp .Results There is a good linear relationship in the range of 0 .007813~1 mg/ml .The average recovery rate is 100 .13% (RSD=3 .02% ) .Conclusion There were large interspecific and intraspecific differences of isorhamnetin-3-rutinoside content in leaves of Nitraria Spp from Xinjiang region .A simple method was identified to give accurate and reproducible results .This method can be used for quantitative analysis of isorhamnetin-3-ru-tinoside content in leaves of N itraria Spp .