The synchronous mechanism of myocardial regeneration-cell disintegration-myocardial fibrosis and its relationship with heart failure / 中华胸心血管外科杂志

ABSTRACT

Objective To observe the cardiomyocytes regeneration after myocardial infarction in Chinese small pigs, ana-lyze the mechanism of myocardial fibrosis after myocardial regeneration. Methods Nine Chinese small pigs, weight 18-20 kg, 6 pigs in the experimental group( to ligate LAD at the equal blood flow point) , and 3 in the control group( no any operation was performed) . Monitor the hemodynamics of the left ventricle. Cardiac specimens were taken after 4 weeks of LAD ligation, the left ventricle was divided into 17 segments, and fixed in 4％ paraformaldehyde for 1 week. Hematoxylin-Eosin/( HE) stai-ning,PTAH、Ki-67-DAB and α-sarcomeric-actin-DAB staining for pathological observation. Results Four weeks of LAD liga-tion, different range of infarct size could be found in all the 17 segment of left ventricle. There was significant systolic pressure difference between the proximal and distal part in the left ventricular cavity. After 4 weeks of LAD ligation, there were a large number of new cardiomyocytes around the infarction area, which connected with the original mature cardiomyocytes directly. Large number of disassembled cardiomyocytes in the infarcted area and myocardial fibers were broken, cell structure disap-peared, and nuclei were scattered in fibrotic tissues. Conclusion New cardiomyocytes after myocardial infarction were derived from the mature cardiomyocytes. Myocardial regeneration, cell disintegration, and myocardial fibrosis were performed synchro-nously under the influence of myocardial tension.