Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
The Korean Journal of Parasitology
;
: 353-355, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-79736
ABSTRACT
There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polymorphisme de restriction
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Cryptosporidiose
/
Cryptosporidium
/
Génotype
Type d'étude:
Etude diagnostique
Limites du sujet:
Enfant
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2013
Type:
Article
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