Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks
Journal of Veterinary Science
; : 379-385, 2004.
Article
Dans En
| WPRIM
| ID: wpr-79775
Responsable en Bibliothèque :
WPRO
ABSTRACT
Potential toxicological interactions of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and/or dibuthyl phthalate (DBP) on ozone were investigated after 32- and 52-wk exposures using hprt mutation assay. Male and female B6C3F1 mice exposed to ozone (0.5 ppm), NNK (1.0 mg/kg), DBP (5,000 ppm), and two or three combinations of these toxicants 6 h per day for 32- and 52-wk showed increases in the frequencies of TG rlymphocytes compared to the control groups. Additive interactions were noted from two combination groups compared to the ozone alone in both sexes of 32- and 52-wk studies. The most common specific mutation type in the hprt genes of test materials-treated male and female mice was transversion with very few transition. The results indicate that such dominant transversion may be responsible for toxicity and combined exposure to ozone, NNK, and DBP induces additive genotoxicities compared to ozone alone.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ozone
/
Analyse de mutations d'ADN
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Cancérogènes
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Lymphocytes T
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RT-PCR
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Phtalate de dibutyle
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Association médicamenteuse
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Hypoxanthine phosphoribosyltransferase
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Tests de mutagénicité
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Mutation
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Veterinary Science
Année:
2004
Type:
Article