Gas Chromatographic Fingerprints of Different Musk / 中国实验方剂学杂志

ABSTRACT

Objective:To establish a gas chromatography(GC) fingerprints analysis method of farmed musk,natural musk and artificial musk for effective control and scientific evaluation of the quality of musk. Method:The sample was split in the 250℃ injection port,with 1:1 split ratio,and separated on a Capillary column(325℃,0.25 mm×30 m,0.25 μm). Nitrogen was used as a carrier gas,the flow rate was 1 mL·min-1. The detector temperature was 280℃. The temperature program was 80℃ for 2 min,increase by 5℃·min-1 to 160℃, and by 1℃·min-1 to 200℃ for 20 min,then 5℃·min-1 to 260℃ for 20 min. GC of the 30 batches of farmed musk,24 batches of natural musk and 8 batches of artificial musk were analyzed by adopting temperature programmer. The similarity evaluation system for chromatographic fingerprint of TCM(2004A)was used to evaluate the similarity of these chromatograms. The fingerprint peaks were identified by using GC-MS combination technology. The ion source temperature was set at 230℃. The quadrupole temperature was set at 150℃. The GC-MS interface temperature was set at 280℃. Electron impact(EI)was used as the ionization source,and the electron energy was set at 70 eV. The scanning scope was m/z 30-550. Result:The GC fingerprints of different kind of musk were established,and the similarities all conformed to regulations. Totally 17 common compounds in farmed musk,10 common compounds in natural musk,and 8 common compounds in artificial musk were determined and compared. Conclusion:This method features a high precision,reproducibility,and stability. The established fingerprints could be used for the overall quality evaluation for musk.