The intervention effect of N-carbamoyl glutamic acid on embryo implantation disorder induced by carbon disulfide and its possible molecular mechanism / 中华劳动卫生职业病杂志

ABSTRACT

Objective@#To explore the preventive effect and possible molecular mechanism of dietary supplementation of N-carbamylglutamate (NCG) in the implantation of carbon disulfide (CS2) into embryo implantation disorders.@*Methods@#embryo implantation disorder model was established by single intraperitoneal exposure to CS2 on the 3rd, 4th, and 5th days after pregnancy. Endometrial tissues were collected for 24h after exposure to CS2 for western-blot and immunohistochemical staining.@*Results@#The number of embryo implantation was increased in NCG+CS2 group, compared with CS2 alone group. Day 4 of pregnancy when CS2-exposed after 24 h, the expression of pAKT protein in NCG+CS2 group was significantly increased (P<0.05), the expression level of pAMPK protein in NCG+CS2 group was significantly decreased, compared with CS2 alone group, respectively. Immunohistochemical results showed that pAKT, pAMPK, AKT and AMPK proteins were expressed in luminal epithelial cells, glandular epithelial cells and stromal cells of endometrium; Day 4 of pregnancy when CS2-exposed after 24 h, deep staining of ATK and pAKT protein in NCG+CS2 group, the AMPK and pAMPK protein staining became lighter.@*Conclusion@#Dietary supplementation of NCG can interfere with the embryo loss induced by CS2 by altering the total amount of AKT/AMPK molecules.