B1 subgenotype of human metapneumovirus detected in children with acute respiratory infection / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To reveal the prevalence of human metapneumovirus (HMPV) among pediatric patients with acute respiratory infection (ARI).@*Methods@#Nasopharyngeal aspirates were collected from hospitalized young children (1-5 years) diagnosed as ARI in Department of Respiratory Disease, Shanxi Provincial Children′s Hospital, Taiyuan city. The nucleoprotein (N) gene of HMPV was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The positive products of PCR were sequenced, made homology and phylogenetic analysis.@*Results@#One sample collected in Feb. 2017 and two samples collected in Nov. 2017 were HMPV positive in RT-PCR detection. The homologies of nucleic acid and deduced amino acid sequences of the three positive PCR products with the corresponding HMPV sequences in GenBank were over 83% and 90%, respectively. Phylogenetic analysis revealed that HMPV B1 subgenotype was detected.@*Conclusions@#HMPV B1 subgenotype was the major epidemic HMPV in young children with ARI in Taiyuan city.