Comparison of the AdvanSure Human Papillomavirus Screening Real-Time PCR, the Abbott RealTime High Risk Human Papillomavirus Test, and the Hybrid Capture Human Papillomavirus DNA Test for the Detection of Human Papillomavirus
Annals of Laboratory Medicine
;
: 201-205, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-80823
ABSTRACT
BACKGROUND:
We evaluated the performance of various commercial assays for the molecular detection of human papillomavirus (HPV); the recently developed AdvanSure HPV Screening real-time PCR assay (AdvanSure PCR) and the Abbott RealTime High Risk HPV PCR assay (Abbott PCR) were compared with the Hybrid Capture 2 HPV DNA Test (HC2).METHODS:
All 3 tests were performed on 177 samples, and any sample that showed a discrepancy in any of the 3 tests was genotyped using INNO-LiPA HPV genotyping and/or sequencing. On the basis of these results, we obtained a consensus HPV result, and the performance of each test was evaluated. We also evaluated high-risk HPV 16/18 detection by using the 2 real-time PCR assays.RESULTS:
Among the 177 samples, 65 were negative and 75 were positive in all 3 assays; however, the results of the 3 assays with 37 samples were discrepant. Compared with the consensus HPV result, the sensitivities and specificities of HC2, AdvanSure PCR, and Abbott PCR were 97.6%, 91.7%, and 86.9% and 83.9%, 98.8%, and 100.0%, respectively. For HPV type 16/18 detection, the concordance rate between the AdvanSure PCR and Abbott PCR assays was 98.3%; however, 3 samples were discrepant (positive in AdvanSure PCR and negative in Abbott PCR) and were confirmed as HPV type 16 by INNO-LiPA genotyping and/or sequencing.CONCLUSIONS:
For HPV detection, the AdvanSure HPV Screening real-time PCR assay and the Abbott PCR assay are less sensitive but more specific than the HC2 assay, but can simultaneously differentiate type 16/18 HPV from other types.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Papillomaviridae
/
Trousses de réactifs pour diagnostic
/
ADN viral
/
Col de l'utérus
/
Sensibilité et spécificité
/
Infections à papillomavirus
/
Papillomavirus humain de type 16
/
Papillomavirus humain de type 18
/
Génotype
Type d'étude:
Etude diagnostique
/
Etude d'étiologie
/
Étude de dépistage
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Annals of Laboratory Medicine
Année:
2012
Type:
Article
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