The polysaccharide isolated from Pleurotus nebrodensis (PN-S) shows immune-stimulating activity in RAW264.7 macrophages / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 355-360, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-812135
ABSTRACT
A novel Pleurotus nebrodensis polysaccharide (PN-S) was purified and characterized, and its immune-stimulating activity was evaluated in RAW264.7 macrophages. PN-S induced the proliferation of RAW264.7 cells in a dose-dependent manner, as determined by the MTT assay. After exposure to PN-S, the phagocytosis of the macrophages was significantly improved, with remarkable changes in morphology being observed. Flow cytometric analysis demonstrated that PN-S promoted RAW264.7 cells to progress through S and G2/M phases. PN-S treatment enhanced the productions of interleukin-6 (IL-6), nitric oxide (NO), interferon gamma (INF-γ), and tumor necrosis factor-α (TNF-α) in the macrophages, with up-regulation of mRNA expressions of interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), interferon gamma(INF-γ) and tumor necrosis factor-α (TNF-α) being observed in a dose-dependent manner, as measured by qRT-PCR. In conclusion, these results suggest that the purified PN-S can improve immunity by activating macrophages.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
ARN messager
/
Cycle cellulaire
/
Régulation positive
/
Lignée cellulaire
/
Interleukine-6
/
Interféron gamma
/
Facteur de nécrose tumorale alpha
/
Pleurotus
/
RT-PCR
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Journal of Natural Medicines (English Ed.)
Année:
2015
Type:
Article
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