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Establishment and reliability evaluation of the design space for HPLC analysis of six alkaloids in Coptis chinensis (Huanglian) using Bayesian approach / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 697-708, 2016.
Article Dans Anglais | WPRIM | ID: wpr-812575
ABSTRACT
Coptis chinensis (Huanglian) is a commonly used traditional Chinese medicine (TCM) herb and alkaloids are the most important chemical constituents in it. In the present study, an isocratic reverse phase high performance liquid chromatography (RP-HPLC) method allowing the separation of six alkaloids in Huanglian was for the first time developed under the quality by design (QbD) principles. First, five chromatographic parameters were identified to construct a Plackett-Burman experimental design. The critical resolution, analysis time, and peak width were responses modeled by multivariate linear regression. The results showed that the percentage of acetonitrile, concentration of sodium dodecyl sulfate, and concentration of potassium phosphate monobasic were statistically significant parameters (P < 0.05). Then, the Box-Behnken experimental design was applied to further evaluate the interactions between the three parameters on selected responses. Full quadratic models were built and used to establish the analytical design space. Moreover, the reliability of design space was estimated by the Bayesian posterior predictive distribution. The optimal separation was predicted at 40% acetonitrile, 1.7 g·mL(-1) of sodium dodecyl sulfate and 0.03 mol·mL(-1) of potassium phosphate monobasic. Finally, the accuracy profile methodology was used to validate the established HPLC method. The results demonstrated that the QbD concept could be efficiently used to develop a robust RP-HPLC analytical method for Huanglian.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Normes de référence / Médicaments issus de plantes chinoises / Chimie / Théorème de Bayes / Chromatographie en phase liquide à haute performance / Coptis / Alcaloïdes / Chromatographie en phase inverse / Méthodes Type d'étude: Étude pronostique langue: Anglais Texte intégral: Chinese Journal of Natural Medicines (English Ed.) Année: 2016 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Normes de référence / Médicaments issus de plantes chinoises / Chimie / Théorème de Bayes / Chromatographie en phase liquide à haute performance / Coptis / Alcaloïdes / Chromatographie en phase inverse / Méthodes Type d'étude: Étude pronostique langue: Anglais Texte intégral: Chinese Journal of Natural Medicines (English Ed.) Année: 2016 Type: Article