Effect of silencing the VDR gene on the migration and invasion of prostate cancer cells / 中华男科学杂志
National Journal of Andrology
;
(12): 969-974, 2017.
Article
Dans Chinois
| WPRIM
| ID: wpr-812848
ABSTRACT
Objective@#To investigate the effect of small interfering RNA silencing the vitamin D receptor (VDR) on the biological behavior of prostate cancer PC-3 cells.@*METHODS@#We constructed the VDR-shRNA lentiviral vector and determined the mRNA and protein expressions of VDR by RT-PCR and Western blot. Using scratch wound healing and Transwell chamber assays, we detected the changes in the migration and invasiveness of the PC-3 cells after silencing VDR.@*RESULTS@#The VDR-shRNA plasmid significantly interfered the VDR expression and successfully screened the cell lines with stable VDR-shRNA interference. The rate of scratch wound healing was markedly lower in the VDR interference group than in the blank control and LV3 negative control groups (59% vs 73.6% and 77.8%, P 0.05), and so was the count of permeable cells (P 0.05). The migration ability and invasiveness of the VDR-treated cells were remarkably decreased as compared with those of the control cells.@*CONCLUSIONS@#Down-regulated expression of the VDR gene may reduce the migration and invasiveness of prostate cancer cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Plasmides
/
Tumeurs de la prostate
/
Cicatrisation de plaie
/
ARN messager
/
Transfection
/
Régulation négative
/
Mouvement cellulaire
/
Récepteur calcitriol
/
Lentivirus
Limites du sujet:
Humains
/
Mâle
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2017
Type:
Article
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