Protective effects of P2X7 receptor inhibition in cerebral ischemia/reperfusion injury in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1169-1176, 2018.
Article
de Zh
| WPRIM
| ID: wpr-813120
Bibliothèque responsable:
WPRO
ABSTRACT
To investigate the protective effects of P2X7 receptor (P2X7R) inhibitor against cerebral ischemia/reperfusion (I/R) injury in rats and the possible mechanisms.
Methods: The neurological deficit of rats was evaluated by Longa score. The infarct volume was examined by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. The expression levels of extracellular signal-regulated kinase (ERK), phosphorylation extracellular signal-regulated kinas p-ERK), connexin 43 (Cx43), Bax, Bcl-2 and cleaved caspase-3 were detected by Western blot.
Results: Compared with sham group, the neurobehavioral score (P<0.05) and cerebral infarct volume (P<0.01) of rats in I/R group was increased. Compared with I/R group, brilliant blue G (BBG, the antagonist of P2X7R) or PD98059 (the inhibitor of EKR kinase) could reduce the neurobehavioral score (P<0.01) and cerebral infarct volume significantly (P<0.05). The neurobehavioral score and cerebral infarct volume was further decreased (P<0.05) when rats were treated with both BBG and PD98059. Compared with I/R group, the expression levels of p-ERK, Cx43, cleaved caspase-3 and the ratio of Bax/Bcl-2 were decreased by BBG or PD98059 pretreatment, and the protective effects were further enhanced when rats were treated with both BBG and PD98059 (P<0.05).
Conclusion: Inhibition of P2X7R reduces the cerebral I/R injury of rats. ERK inhibition is probably involved in the protective effects of P2X7R inhibitor against cerebral I/R injury, which may be related to the reduction of Cx43 and cleaved caspase-3, and the ratio of Bax/Bcl-2.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Phosphorylation
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Lésion d'ischémie-reperfusion
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Encéphalopathie ischémique
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Régulation de l'expression des gènes
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Utilisations thérapeutiques
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Traitement médicamenteux
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Récepteurs purinergiques P2X7
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Antagonistes des récepteurs purinergiques P2X
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2018
Type:
Article