Comparision of several different peritoneal fibrosis rat models / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 162-166, 2005.
Article
de Zh
| WPRIM
| ID: wpr-813413
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To compare different types of peritoneal fibrosis models in rats.@*METHODS@#Thirty-four SD rats were divided into 5 groups: control group (Group 1), normal saline group (Group 2), high glucose group (4.25% peritoneal dialysate, 4.25% PDF, Group 3), high glucose + lipopolysaceharides (LPS) group (4.25% PDF + LPS, Group 4), high glucose + erythromycin group (4.25% PDF + lactobionate erythromycin, Group 5). A 2-hour peritoneal equilibration test (PET) was performed after 5 weeks. Then animals were humanely killed. Dialysate-to-plasma urea ratio (D/ Purea), glucose reabsorption (D2/D0), net ultrafiltration (UF) volume were determined. The level of fibronectin in peritoneal tissues was measured by immunohistochemical method. Peritoneal membrane histology was evaluated by light microscopy.@*RESULTS@#The D2/D0 ratio and net ultrafiltration volume in Groups 3, 4, and 5 were significantly lower than those in Groups 1 and 2 (P < 0.05) . The D/Purea ratio in Groups 3, 4 and 5 were significantly higher than that in Groups 1 and 2 (P < 0. 05 ). The level of fibronectin in Groups 3, 4 and 5 were significantly higher than that in Groups 1 and 2 (P < 0.05 ).@*CONCLUSION@#Different types of peritoneal fibrosis models in rats has been established. The best model is high clusion glucose + erythromycin.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
/
Péritoine
/
Fibrose
/
Répartition aléatoire
/
Dialyse péritonéale
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Rat Sprague-Dawley
/
Modèles animaux de maladie humaine
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2005
Type:
Article