Effects of advanced glycation end products on the activity of NF-kappaB and the expression of fibronectin mRNA in the endothelial cells in aged rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 883-887, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-813581
ABSTRACT
OBJECTIVE@#To explore the effects of advanced glycation end products (AGEs) on the activity of NF-kappaB and fibronectin (Fn) synthesis in the endothelial cells in aged rats.@*METHODS@#Endothelial cells were cultured in M199 from the aorta of 24 month old rats and divided into 3 groups Group A (5 mmol/L glucose) as controls, Group B (25 mg/L AGEs for 48 h), and Group C (50 mg/L AGEs for 48 h). The activity of NF-kappaB was evaluated by immunofluorescence and the expression of Fn mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR).@*RESULTS@#Compared with the controls, AGEs induced the activity of NF-kappaB and increased the Fn mRNA expression in a concentration-dependent manner (P<0.05).@*CONCLUSION@#The activity of NF-kappaB and up-regulated expression of Fn mRNA induced by AGEs may contribute to chronic complications of diabetes.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
ARN messager
/
Cellules cultivées
/
Facteur de transcription NF-kappa B
/
Fibronectines
/
Rat Sprague-Dawley
/
Produits terminaux de glycation avancée
/
Cellules endothéliales
/
Génétique
/
Métabolisme
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2006
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS