Killing effect of VP3 on human nasopharyngeal carcinoma cell line CNE-2 cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 706-709, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-813616
ABSTRACT
OBJECTIVE@#To investigate the killing effects of VP(3) on nasopharyngeal carcinoma cell line CNE-2.@*METHODS@#Plasmid expression vector pcDNA3.1(-) CMV.VP(3)-His was constructed and identified by Kpn I/EcoR I endonuclease analysis, and then sequenced to verify successful insertion in the sense direction of VP(3) gene. pcDNA3.1(-) CMV.VP(3)-His and pcDNA3.1(-)-His expression plasmid was transiently transfected into nasopharyngeal carcinoma cell line CNE-2 . VP(3) protein expression was detected by Western blotting. MTT assay was used to determine the killing effects of VP(3) gene on nasopharyngeal carcinoma cell line CNE-2.@*RESULTS@#Endonuclease analysis and sequencing confirmed the recombinant plasmid contained the complete VP(3) CDS sequence. Western blotting detected a 14.03 kD protein expression from the transfected cells, which was the expecting band of VP(3) gene. The growth of CNE-2 cells that expressed VP(3) gene was inhibited,while the growth of CNE-2 cells that did not express VP(3) gene was not inhibited.@*CONCLUSION@#VP(3) gene can kill nasopharyngeal carcinoma cell CNE-2.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Physiologie
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Transfection
/
Thérapie génétique
/
Tumeurs du rhinopharynx
/
Protéines de capside
/
Lignée cellulaire tumorale
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2006
Type:
Article
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