Screening for proteins interacting with ataxin-3, the gene product of SCA3/MJD / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 40-44, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-813770
ABSTRACT
OBJECTIVE@#To screen for proteins interacting with ataxin-3 by yeast two-hybrid system 3, and to discuss the function of ataxin-3 and pathogenesis of spinocerebellar ataxia type 3 and Machado-Joseph disease (SCA3/MJD).@*METHODS@#First we sub-cloned the full reading frame of both wild-type and mutant ataxin-3 into carrier pGBKT7 (ataxin-3-bait), and then screened human brain cDNA library with ataxin-3-bait.@*RESULTS@#We found five positive clones in 6.5 x 10(6) transformers. After sequencing, we knew all of them were novel ataxin-3 interacting proteins. Three were corresponded to the known sequences coding the known proteins, which were human Rho GDP dissociation inhibitor alpha, small ubiquitin-like modifier 1, and human neuronal amiloride-sensitive cation channel 2. Another two of the five were unknown.@*CONCLUSION@#Small ubiquitin-like modifier 1 probably interacted with ataxin-3, suggesting that the sumoylation probably participated in post-translation modifying of ataxin-3 and pathogenesis of SCA3/MJD.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines de répression
/
Levures
/
Encéphale
/
Protéines nucléaires
/
Dégénérescences spinocérébelleuses
/
Banque de gènes
/
Techniques de double hybride
/
Cartographie d'interactions entre protéines
/
Ataxine-3
/
Génétique
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2006
Type:
Article
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