Apoptosis of mesenchymal cell line MBA-1 induced by core binding factor alpha 1 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 14-18, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-813776
ABSTRACT
OBJECTIVE@#To investigate the effect of two core binding factors alpha 1 (Cbfa1) isfroms (Cbfa1/P56 and Cbfa1/P57) on the apoptosis of mesenchymal cell line MBA-1.@*METHODS@#The two Cbfal isfroms were transiently transfected into MBA-1 cells, then the changes of apoptosis rate were observed by flow cytometer. The protein expressions of Cbfa1, Bax, Bcl-2, caspase-3, and caspase-9, cytochrome-C and TNF-alpha were determined by Western immunoblot.@*RESULTS@#After the transient transfection with the two isforms of Cbfa1, MBA-1, the cells apoptotic rates increased, and the ratio of Bax/Bcl-2, the expressions of cytochrome-C, caspase-3, caspase-9, and TNF-alpha were significantly increased.@*CONCLUSION@#Cbfa1 can promote the apoptosis in mesenchymal cell line MBA-1. Bax/Bcl-2, cytochrome-C, caspase-9, caspase-3, and TNF-alpha are also involved in the apoptosis pathway.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Physiologie
/
Cellules de la moelle osseuse
/
Lignée cellulaire
/
Apoptose
/
Protéines proto-oncogènes c-bcl-2
/
Caspases
/
Isoformes de protéines
/
Biologie cellulaire
/
Cytochromes c
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2006
Type:
Article
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