Proteomic study of paclitaxel on human cervical carcinoma HCE1 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 512-517, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-814047
ABSTRACT
OBJECTIVE@#To explore the mechanism of paclitaxel on the protein expression of human cervical carcinoma cell line HCE1.@*METHODS@#The total proteins extracted from paclitaxel-treated HCE1 cells were analyzed by 2-dimensional gel electrophoresis (2-DE), and compared with those from untreated HCE1 cells. The differential proteins were identified by mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.@*RESULTS@#At 24 hour after paclitaxel (0.05 mumol/L) treatment, 2-DE images of paclitaxel-treated and paclitaxel-untreated cells were analyzed. Forty-two differential proteins were found. Twenty-one differential proteins among 42 proteins were analyzed by mass spectrometry, among which 15 proteins were identified, including peptidyl-prolylisomerases A (PPIase A),alpha-enolase,keratin 8,heat shock protein 90, eukaryotic translation initiation factor 1A, and so on.@*CONCLUSION@#Fifteen proteins in human cervical carcinoma cells paclitaxel-treated and paclitaxel-untreated are found by proteomic techniques. These proteins may be involved in the proliferation inhibition of human cervical carcinoma cells by paclitaxel.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Enolase
/
Cellules cancéreuses en culture
/
Marqueurs biologiques tumoraux
/
Facteur-1 d'initiation eucaryote
/
Tumeurs du col de l'utérus
/
Génome
/
Paclitaxel
/
Protéome
Type d'étude:
Étude pronostique
Limites du sujet:
Femelle
/
Humains
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2008
Type:
Article
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