Changes of nucleolin expression and cellular localization during HUVEC apoptosis induced by hydrogen peroxide / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 488-493, 2008.
Article
de Zh
| WPRIM
| ID: wpr-814051
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the expression and cellular localization of nucleolin C23 during human umbilical vein endothelial cell (HUVEC) apoptosis induced by hydrogen peroxide (H(2)O(2)).@*METHODS@#Apoptosis of HUVEC was induced by exposure to 0.5 mmol/L H(2)O(2) for different periods and detected by flow cytometry and activity of caspase-3. The mRNA and protein expression of nucleolin were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot, respectively. The intracellular distribution of nucleolin was observed by indirect immunofluorescence.@*RESULTS@#The percentage of apoptotic cells was increased significantly after treatment with H(2)O(2) for 12, 24 and 36 hours. The activity of caspase-3 reached the peak after treatment with H(2)O(2) for 4 h. RT-PCR showed that nucleolin C23 mRNA was decreased after 2, 4, and 8 hours treatment with H(2)O(2). Western blot showed that C23 protein level was decreased after 12 hours with an additional cleft band of 80 kD appeared after 8 hours. Density analysis showed that the 80 kD cleft band increased in a time-dependent manner. Immunofluorescence analysis demonstrated that H(2)O(2)-induced C23 redistribution from the nucleus to the cytoplasm.@*CONCLUSION@#H(2)O(2) could induce apoptosis accompanying with C23-cleavage and C23-translocation from the nucleus to the cytoplasm.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Phosphoprotéines
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Veines ombilicales
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ARN messager
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Noyau de la cellule
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Cellules cultivées
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Protéines de liaison à l'ARN
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Apoptose
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Biologie cellulaire
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Cytoplasme
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2008
Type:
Article