Construction of eukaryotic expression vector encoding human nasopharyngeal carcinoma anti-idiotype antibody single chain fragment gene G22 and its expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 16-20, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-814131
ABSTRACT
OBJECTIVE@#To construct a eukaryotic expression vector encoding human nasopharyngeal carcinoma anti-idiotype antibody single chain fragment (ScFv) gene G22, and to identify its expression in rectal cancer cells (CMT-93).@*METHODS@#The G22 gene was ligated into the sites of EcoRI and NotI of eukaryotic expression vector pcDNA3.1(+). After the identification and DNA sequencing, the recombinant plasmid pc DNA3.1(+)-G22 was stably transfected into CMT-93 cells, and the expression of G22 was detected by Western blot, flow cytometry and immunofluorescence staining.@*RESULTS@#Restriction enzyme digestion and DNA sequencing showed that the recombinant plasmid contained the human nasopharyngeal carcinoma anti-idiotype antibody ScFv gene G22. Transfection experiment verified that G22 gene could be expressed in CMT-93 cells in the right way.@*CONCLUSION@#The eukaryotic expression vector containing the human nasopharyngeal carcinoma anti-idiotype antibody ScFv gene G22 is successfully constructed and expressed, which is the basis for further study of its DNA vaccine.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines recombinantes
/
Région variable d'immunoglobuline
/
Tumeurs du rhinopharynx
/
Anticorps anti-idiotypiques
/
Allergie et immunologie
/
Cellules eucaryotes
/
Vecteurs génétiques
/
Génétique
/
Métabolisme
/
Anticorps antitumoraux
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2008
Type:
Article
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