Preparation and preliminary application of colloidal carbon dipstick for schistosomiasis japonica / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1063-1069, 2009.
Article
Dans Anglais
| WPRIM
| ID: wpr-814192
ABSTRACT
OBJECTIVE@#To develop a rapid and simple immunoassay to detect antibodies in the sera of patients infect Schistosoma japonicum (S. japonicum).@*METHODS@#Soluble egg antigen (SEA) of S. japonicum conjugated with colloidal carbon in advance was used to react with the antibodies in the sera of patients with schistosomiasis. Then the carbon-antigen-antibody complex would be captured by SEA which had been absorbed on the nitrocellulose membrane and a gray band was shown.@*RESULTS@#A total of 137 sera samples from S. japonicum epidemic area were tested, and the consistency, sensitivity, and specificity of colloidal carbon dipstick assay were 98.54%, 98.99%, and 97.37%, respectively, compared with the IHA method. The gray scale of bands on the dipstick was curvilinear to serum titer which revealed that the assay could be used semi-quantitatively in serum analysis.@*CONCLUSION@#Colloidal carbon dipstick assay is not only rapid and simple, but also sensitive and specific for the detection of serum antibodies of schistosomiasis japonica. It will be a practical immunological assay for the diagnosis of schistosomiasis in the field testing.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Schistosoma japonicum
/
Sang
/
Schistosomiase artérioveineuse
/
Dosage immunologique
/
Carbone
/
Anticorps antihelminthe
/
Chimie
/
Sensibilité et spécificité
/
Colloïdes
/
Diagnostic
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2009
Type:
Article
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