SARA expression in high glucose induced epithelium to mesenchymal transition of proximal tubule cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1230-1235, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-814340
ABSTRACT
OBJECTIVE@#To construct the cell model of epithelium to mesenchymal transition of proximal tubule cells induced by high glucose and to determine the expression of Smad anchor for receptor activation (SARA).@*METHODS@#Protein expression of vimentin, Zona occludens-1(ZO-1), and SARA was determined by Western blot, and their mRNA expressions were detected by Real-time PCR.@*RESULTS@#After stimulation by 30 mmol/L D-glucose, the protein and mRNA expression levels of vimentin in HK-2 cells increased in a time-dependent manner while the expression of ZO-1 was reduced significantly, especially at 48 h. Meanwhile, SARA was also decreased in a time-dependent manner.@*CONCLUSION@#High glucose can induce renal epithelium to mesenchymal transition, and SARA may be involved in this process as a protector.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Phosphoprotéines
/
Vimentine
/
ARN messager
/
Serine endopeptidases
/
Transduction du signal
/
Différenciation cellulaire
/
Cellules cultivées
/
Biologie cellulaire
/
Protéines et peptides de signalisation intracellulaire
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2010
Type:
Article
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