Limb ischemic preconditioning reduces rabbit hepatic ischemia-reperfusion injury through inhibition the phosphorylation of proteins in the MAPK signal pathway in the late phase / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 591-597, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-814622
ABSTRACT
OBJECTIVE@#To investigate the liver protection mechanisms of MAPK signaling pathway of limb ischemia preconditioning in the late phase.@*METHODS@#Thirty-six adult male New Zealand white rabbits, weighing 1.8-2.0 kg, were randomly divided equally into 3 groups group C (sham operation), group L (liver ischemia-reperfusion 24 h after limb ischemia preconditioning), group IR (liver ischemia-reperfusion without limb ischemia preconditioning). Serum alanine transaminase (ALT) was measured during ischemia reperfusion. The tissue and cell injury of liver were examined by optical and electron microscopy. Activation of P38MAPK, P44/P42MAPK, and JNK in hepatic tissue was assessed by western blot after 30 min of reperfusion.@*RESULTS@#Serum ALT and cell injury in the liver as examined by optical and electron microscopy was decreased in group L as compared with the group IR. Phosphorylation of P38MAPK, P44/ P42MAPK, and JNK were all increased significantly after 30 min of reperfusion. Phosphorylation of P38MAPK and JNK was reduced by limb ischemia pre-treatment.@*CONCLUSION@#Limb ischemia pre-treatment can induce the late phase of preconditioning in rabbit liver through the inhibition of the phosphorylation of P38MAPK and JNK.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation
/
Physiologie
/
Lésion d'ischémie-reperfusion
/
Chimie
/
Préconditionnement ischémique
/
Système de signalisation des MAP kinases
/
P38 Mitogen-Activated Protein Kinases
/
Membres
/
Foie
/
Méthodes
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2012
Type:
Article
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