Screening of membrane antigen differentially expressed in androgen-dependent prostate cancer and androgen-independent prostate cancer / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 817-823, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-814789
ABSTRACT
OBJECTIVE@#To determine the screening of the expression of membrane proteins in androgen-dependent prostate cancer (ADPC) and androgen-independent prostate cancer (AIPC) and to explore the mechanism of membrane proteins in these two cancers.@*METHODS@#Serum samples were collected from 3 patients with ADPC and another 3 patients with AIPC. The serum was incubated with ADPC cell line LNCaP and/or AIPC cell line PC-3 and detected by immunoprecipitation and Western blot. Differentially expressed proteins between ADPC and AIPC identified by mass spectrometry were compared and their expression level and location were analyzed by immunofluorescence.@*RESULTS@#Altogether 11 membrane proteins were identifited, such as the Neural-Cadherin precursor, ER60 precursor, Claudin-4, and so on. Immunofluorescence revealed that the expression level of Claudin-4 in PC-3 cells was higher than in LNCaP cells.@*CONCLUSION@#We can use the screening method to study membrane proteins in prostate cancer. Claudin-4 may play an important role in the pathogenesis and the development of AIPC.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Tumeurs de la prostate
/
Glycoprotéines membranaires
/
Protein Disulfide-Isomerases
/
Analyse de profil d'expression de gènes
/
Lignée cellulaire tumorale
/
Claudine-4
/
Génétique
/
Androgènes
/
Métabolisme
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Adulte très âgé
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2012
Type:
Article
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