Efficacy of bispecific targeted immunotoxin DTATEGF against NSCLC brain metastatic tumor PC9-BrM3 cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1217-1222, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-814838
Responsable en Bibliothèque :
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ABSTRACT
OBJECTIVE@#To investigate the in vitro and in vivo anticancer efficacy of the immunotoxin DTATEGF against human NSCLC brain metastatic tumor PC9-BrM3 cell line.@*METHODS@#The effect of the immunotoxin DTATEGF was tested for its ability to inhibit the proliferation of PC9-BrM3 cells in vitro by MTT assay. The cell cycle and the apoptosis of cells with 1 pmol/L DTATEGF were examined by flow cytometry. In vivo, 2 μg of DTATEGF or control Bickel3 was given intratumor to nude mice with established PC9-BrM3 xenografts on their hips, and tumor volumes were measured and tumor samples were investigated by immunchistochemistry SABC method. The microvessel density (MVD) was measured in each group.@*RESULTS@#In vitro, DTATEGF killed PC9-BrM3 cells and showed an IC50 of 1 pmol/L. The apoptotic rate in the 1 pmol/L DTATEGF group was (64.0±0.5)% , significantly higher than that in the control group (1.5±0.4)% (P<0.01). The cell cycle was obviously inhibited by DTATEGF in a dose-dependent manner. The percentage of cells treated with 1 pmol/L DTATEGF in SubG0/G1 phase was (32.0±1.5)%, significantly higher than that in the control group (2.0±0.4)% (P<0.01). In vivo, DTATEGF significantly inhibited the growth of PC9-BrM3 hip tumors (P<0.05). The MVD of the DTATEGF group was (15.6±4.6)/mm2, significantly lower than that of the control group (31.2±5.4)/mm2 (P<0.01).@*CONCLUSION@#DTATEGF inhibits the growth of the PC9-BrM3 cell line and induces its apoptosis. It is highly efficacious against human metastatic NSCLC brain tumor and against neovascularization.
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Tumeurs du cerveau
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Cycle cellulaire
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Immunotoxines
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Apoptose
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Anticorps bispécifiques
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Carcinome pulmonaire non à petites cellules
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Tests d'activité antitumorale sur modèle de xénogreffe
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Lignée cellulaire tumorale
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Traitement médicamenteux
Limites du sujet:
Animals
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Humans
langue:
Zh
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2013
Type:
Article