p16 gene CpG methylation in cervical squamous cell carcinoma and HPV16 infection in Uyghur women / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 48-53, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-814933
ABSTRACT
OBJECTIVE@#To determine the correlation between p16 gene CpG methylation sites in the promoter region and HPV16 infection in cervical squamous cell carcinoma in Xinjiang Uyghur women.@*METHODS@#MALDI-TOF MS was used quantitatively to analyze p16 gene promotor methylation status of CpG islands in 20 cervix squamous cell carcinomas and 20 corresponding non-cancerous tissues in Uyghur women. HPV16 infection was detected by polymerase chain reaction (PCR) in both groups.@*RESULTS@#Among the 16 CpG sites in the p16 gene promoter region, CpG1-2 and CpG 6 sites were different between the 2 groups, and the levels of CpG1-2 and CpG6 methylation sites in the cervical squamous cell carcinoma were higher than those in the control group. The presence of HPV16 infection was significantly different between the cervix squamous cell carcinoma tissue and non-cancerous tissues (P<0.05). There was no significant correlation between p16 gene CpG methylation sites and HPV16 infection of cervical squamous cell carcinoma in Uyghur women.@*CONCLUSION@#P16 gene CpG 1-2, CpG 6 hypermethylation and HPV16, which are independent of one another, play an important role in cervical squamous cell carcinogenesis in Uyghur women.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Carcinome épidermoïde
/
Tumeurs du col de l'utérus
/
Chine
/
Régions promotrices (génétique)
/
Ilots CpG
/
Méthylation de l'ADN
/
Gènes p16
/
Infections à papillomavirus
/
Papillomavirus humain de type 16
Limites du sujet:
Femelle
/
Humains
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2013
Type:
Article
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