Screening and functional analysis of microRNA expression in HPV16-positive squamous carcinoma of the cervix
in the Uygur of southern Xinjiang / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 701-709, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-815283
ABSTRACT
OBJECTIVE@#To explore the differential expression of microRNA (miRNA) in HPV16-positive squamous carcinoma of the cervix in the Uygur of southern Xinjiang and to predict the target genes of the miRNAs.
@*METHODS@#Samples of HPV16-positive squamous carcinoma of the cervix from 5 Uygurs were collected for miRNA microarray assay. The differentially expressed miRNAs were selected for further verification by real-time quantitative RT-PCR. The software, including targetscan, miRwalk, miRanda and pictar, were used to predict the target genes of the verified miRNAs.
@*RESULTS@#Eighteen differentially expressed miRNAs were identified by miRNA microarray assay. The significantly differentially expressed miRNA-138 and miRNA-720 were verified by real-time quantitative RT-PCR. According to the prediction, the target genes for miRNA-138 were EZH2, LYPLA1, ARHGEF3, CLNS1A, EIF4EBP1, GNAI2, LIMK1, RHOC, ROCK2, SLC20A1, TERT, and H2AFX, while for miRNA-720 were EZH2, AGAP2, SPOCK2, FGF14, HNRNPA2B1, QKI, FOXG1, ACVR1B, DNMT3A, EPHB2, LATS2, KRAS, CCND2, NBN, ENAM, AMELX, PRNP, and CALB1.
@*CONCLUSION@#miR-138 and miR-720 are the down-regulated target miRNAs in HPV16-positive squamous carcinoma of the cervix in the Uygur of southern Xinjiang. The common target gene for miR-138 and miR-720 is EZH2, which might be related to cervical squamous carcinoma invasion and metastasis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Carcinome épidermoïde
/
Régulation négative
/
Tumeurs du col de l'utérus
/
Chine
/
Infections à papillomavirus
/
MicroARN
/
Papillomavirus humain de type 16
/
Réaction de polymérisation en chaine en temps réel
/
Métabolisme
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Femelle
/
Humains
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Journal of Central South University(Medical Sciences)
Année:
2015
Type:
Article
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