Study on the Effects of Tetrandrine on Proliferation and Migration ,Invasion Ability of Neuroblastoma Cells and Its Mechanism / 中国药房
China Pharmacy
; (12): 211-216, 2019.
Article
de Zh
| WPRIM
| ID: wpr-816723
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WPRO
ABSTRACT
OBJECTIVE: To investigate the effects of tetrandrine (TET) on the proliferation and migration, invasion ability of neuroblastoma cells and its mechanism. METHODS: Human neuroblastoma cell lines IMR-32 and SH-SY5Y were chosen as objects. MTT assay was used to detect the effects of 0 (blank control), 2.5, 5, 10, 15, 20 μmol/L TET on cell proliferation. The transmembrane number of normal control group (TET concentration of 0 μmol/L) and TET group (10 μmol/L for IMR-32 cells, 15 μmol/L for SH-SY5Y cells) were investigated by Transwell cell migration and invasion experiments. The protein levels of MMP-2, MMP-9, β-catenin, GSK3β and p-GSK3β in IMR-32 cells and SH-SY5Y cells were tested by Western blotting assay after treated with TET of above concentrations. RESULTS: TET with 5, 10, 15, 20 μmol/L can reduce the survival rate of IMR-32 cells and SH-SY5Y cells significantly (P<0.05 or P<0.01). IC50 of which to IMR-32 cells and SH-SY5Y cells were 10.148 and 14.461 μmol/L, respectively. Results of migration and invasion experiments showed that compared with normal control group, the number of transmembrane cells was decreased significantly in TET group (P<0.01). Results of Western blotting assay showed that compared with normal control group, the protein expression levels of MMP-2,MMP-9,β-catenin and p-GSK3β were decreased significantly in TET group (P<0.01). CONCLUSIONS: TET shows significant inhibitory effects on the proliferation, migration and invasion ability of neuroblastoma cells, the mechanism of which may be associated with down-regulating the protein expression of MMP-2 and MMP-9 and inhibiting Wnt/β-catenin signaling pathway.
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WPRIM
langue:
Zh
Texte intégral:
China Pharmacy
Année:
2019
Type:
Article