Inhibition of Pim-1 attenuates the proliferation and migration in nasopharyngeal carcinoma cells
Asian Pacific Journal of Tropical Medicine
;
(12): 645-650, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-819603
ABSTRACT
OBJECTIVE@#To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma (NPC) cells.@*METHODS@#Pim-1 expressions in NPC cell lines CNE1, CNE1-GL, CNE-2Z and C666-1 were examined by RT-PCR, western blotting and immunoflucesence, respectively. After CNE1, CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor, quercetagetin, the cell viability, colony formation rate and migration ability were analyzed.@*RESULTS@#Pim-1 expression was negative in well-differentiated CNE1 cells, whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells. Interestingly, CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1. Treatment of CNE1-GL and C666-1 cells with quercetagetin significantly decreased the cell viability, colony formation rate and migration ability but not the CNE1 cells.@*CONCLUSIONS@#These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration, and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Carcinomes
/
Test clonogénique de cellules souches tumorales
/
Marqueurs biologiques tumoraux
/
Mouvement cellulaire
/
Survie cellulaire
/
Tumeurs du rhinopharynx
/
Technique de Western
/
4H-1-Benzopyran-4-ones
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Asian Pacific Journal of Tropical Medicine
Année:
2012
Type:
Article
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