Profiles of Entamoeba histolytica-specific immunoglobulins in human sera
Asian Pacific Journal of Tropical Medicine
;
(12): 234-238, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-819792
ABSTRACT
OBJECTIVE@#To determine the profiles of anti-Entamoeba histolytica (E. histolytica) IgA, IgG, and IgM in sera of diarrheic and non-diarrheic individuals and partially characterize target antigens.@*METHODS@#Serum samples from thirty diarrheic and thirty non-diarrheic individuals were subjected to IgA, IgG, and IgM profiling through enzyme-linked immunosorbent assay (ELISA), flow cytometry, and immunoblot.@*RESULTS@#ELISA titer results showed that both diarrheic and non-diarrheic individuals possess high levels of E. histolytica-specific IgG compared to IgA and IgM. Flow cytometry data showed that diarrheic serum samples had higher mean reaction percentages against E. histolytica cells compared to non-diarrheic samples. Immunoreactive E. histolytica proteins with molecular weights ranging between 7 kDa and 292 kDa were recognized by diarrheic serum IgG, and 170 kDa and 250 kDa by non-diarrheic serum IgG.@*CONCLUSIONS@#Our findings suggest that serum anti-E. histolytica IgG, compared with serum anti-E. histolytica IgA and IgM responses, was generally high in both diarrheic and non-diarrheic sera, indicating a past exposure to the organism both in symptomatic patients as well as in asymptomatic carriers, respectively. In addition, serum IgG from diarrheic and non-diarrheic patients were able to detect immunogenic E. histolytica proteins.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Sang
/
Immunoglobuline A
/
Immunoglobuline G
/
Immunoglobuline M
/
Immunoglobulines
/
Test ELISA
/
Anticorps antiprotozoaires
/
Immunotransfert
/
Diarrhée
/
Entamoeba histolytica
Limites du sujet:
Adulte
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Asian Pacific Journal of Tropical Medicine
Année:
2012
Type:
Article
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