Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 732-739, 2020.
Article
Dans Chinois
| WPRIM
| ID: wpr-826903
ABSTRACT
We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA. The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/μL). Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40. The sensitivity of this method was satisfactory. The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2. Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis. Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pneumopathie virale
/
Normes de référence
/
Facteurs temps
/
Virologie
/
ARN viral
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Infections à coronavirus
/
Diagnostic précoce
/
Diagnostic
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2020
Type:
Article
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