Effect of Chidamide on the Killing Acitivity of NK Cells Targeting K562 Cells and Its Related Mechanism In Vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1167-1170, 2020.
Article
de Zh
| WPRIM
| ID: wpr-827145
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of chidamide on the killing activity of NK (Natural killer cell, NK) cells targeting K562 cells and its related mechanism.@*METHODS@#K562 cells were pretreated with chidamide at different concentrations and cocultured with NK cells at different effect-target ratios. The killing effect of chidamide on K562 cells by NK cells, the expression of natural killer group 2 member D (NKG2D) ligands and apoptosis rate of K562 cells were detected by flow cytometry.@*RESULTS@#The killing sensitivity of NK cells to K562 cells could be enhanced by chidamide. The expression of ULBP2 on K562 cell surface could be up-regulate, however, the expression of ULBP1 and MICA/MICB showed no statistically difference as compared with control group. Chidamide showed no obvious cytotoxicity to K562 cells.@*CONCLUSION@#Chidamide can significantly improve killing efficiency of NK cells on K562 cells, which may be related to the up-regulation of ULBP2 expression.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Benzamides
/
Cellules tueuses naturelles
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Antigènes d'histocompatibilité de classe I
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Cellules K562
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Protéines et peptides de signalisation intercellulaire
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Allergie et immunologie
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Sous-famille K des récepteurs de cellules NK de type lectine
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Protéines liées au GPI
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Aminopyridines
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2020
Type:
Article