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Comparison of B-NDG and BALB/c mouse models bearing patient-derived xenografts of esophageal squamous cell carcinoma / 南方医科大学学报
Journal of Southern Medical University ; (12): 1200-1206, 2020.
Article Dans Chinois | WPRIM | ID: wpr-828908
ABSTRACT
OBJECTIVE@#To investigate the difference of tumor formation in different mouse strains bearing patient-derived xenograft of esophageal squamous cell carcinoma(ESCC) and establish a better animal model for preclinical study of individualized treatment of ESCC.@*METHODS@#The tumor tissues collected from 22 ESCC patients were used to establish tumor-bearing mouse models in B-NDG (NSG) mice and BALB/c nude mice. The tumor formation rate and tumor formation time were compared between the two mouse models, and HE staining, immunohistochemistry and genome sequencing were carried out to assess the consistency between transplanted tumor tissues in the models and patient-derived tumor tissues.@*RESULTS@#The tumor-bearing models were established successfully in both NSG mice (50%, 11/22) and BALB/c nude mice (18.18%, 4/22). The average tumor formation time was significantly shorter in NSG mice than in BALB/c nude mice (75.95 91.67 days, < 0.001). In both of the mouse models, the transplanted tumors maintained morphological characteristics identical to those of patient-derived ESCC tumors. Genetic analysis showed that the xenografts in NSG mice had a greater genetic similarity to the patients' tumors than those in BALB/c nude mice ( < 0.0001).@*CONCLUSIONS@#Mouse models bearing xenografts of patient-derived ESCC can be successfully established in both NSG mice and BALB/c nude mice, but the models in the former mouse strain can be more reliable.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs de l&apos;oesophage / Tests d&apos;activité antitumorale sur modèle de xénogreffe / Lignée cellulaire tumorale / Prolifération cellulaire / Hétérogreffes / Carcinome épidermoïde de l&apos;oesophage / Souris de lignée BALB C / Souris nude Type d'étude: Étude pronostique Limites du sujet: Animaux / Humains langue: Chinois Texte intégral: Journal of Southern Medical University Année: 2020 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Tumeurs de l&apos;oesophage / Tests d&apos;activité antitumorale sur modèle de xénogreffe / Lignée cellulaire tumorale / Prolifération cellulaire / Hétérogreffes / Carcinome épidermoïde de l&apos;oesophage / Souris de lignée BALB C / Souris nude Type d'étude: Étude pronostique Limites du sujet: Animaux / Humains langue: Chinois Texte intégral: Journal of Southern Medical University Année: 2020 Type: Article