Preparation and characteristics of drug loaded PLGA chitosan/nano-hydroxyapatite membrane for guided periodontal tissue regeneration in surgical implanting / 第二军医大学学报

ABSTRACT

Objective To prepare the porous drug-conlaining membrane by poly (ladic-co-glycolic acid) (PLGA)/ chitosan (CS)/nano-hydroxyapatitc for guided periodontal tissue regeneration in surgery, and lo evaluate its performance in vitro. Methods The samples were divided into four groups by different mass ratios of the PLGA lo CS (100: 0, 90: 10, 80: 20 and 70: 30). The PLGA/CS/nano-hydroxyapatitcporous films were prepared by freeze-drying process, wilh polyvinylpyrrolidone (PVP) used as porogcn. The best ratio was chosen by detecting the porosity, water absorption, mechanical properties and degradation of the films; and then il was used as drug carrier lo prepare membrane material for clindamycin controlled release. The morphology of membrane was observed by scan electronmicroscope, (he porosity was detected by anhydrous ethanol liquid displacement method, water absorption was determined by ratio of wet to dry weight of the film, (he wel mechanical performance was tested by electronic universal material testing, (he degradation was determined by weight loss and swelling degree, and the release character was investigated by ultraviolet spcctrophotometric method. In the in vitro experiments, periodontal ligament fibroblast cells (PLFs) were cultured in the membrane for 1-7 days, and cell proliferation was measured by CCK-8. Results The optimal porosity and degradation were found when the mass ratio of PLGA lo CS was 90: 10, wilh the porosity being (28. 66il. 35)%, water absorption being (108. 65 ± 2. 27) %, tensile strength being (2. 36 ± 0. 04) MPa, elongation at break of films being (203.64±3. 89)%, breaking power being (45. 98 ± 2. 46) N, and degradation being (17. 60 ± 0. 86)%. The maximum drug release was 150 μg • mL-1 • d-1, and the effective drug release concentration lasted for 15 d, which could promote the proliferation of PLFs. Conclusion The porous PLGA/CS/nano-hydroxyapatite film prepared in the present study has optimal porosity, its degradation in vitro fit well with the tissue growth, and can create and maintain a specific space for guided periodontal tissue regeneration, allowing for steady drug release for a certain period of time.