MicroRNA-484 regulates liver fibrosis course through targeting Fisl in hepatic stellate cells / 第二军医大学学报

ABSTRACT

Objective To explore the effects of microRNA-484 (miR-484) on hepatic stellate cells (HSC's), the key cell in the occurrence and development of liver fibrosis, and to investigate the functions. Methods On the basis of our previous microarray analysis results, we transfected HSC-T6 cell lines with miR-484 inhibitor to intervene the expression of miR-484 in vitro. The expressions of mRNA and proteins related to liver fibrosis and apoptosis were detected by qPCR and Western blotting, respectively. The cell apoptosis with Annexin V-FITC/PI double staining was determined by flow cytometry. Results Compared with the control group, the mRNA and protein expression of miR 484-targeted gene Fisl and proapoptotic factor caspase-3 were both significantly up-regulated (P<0. 05), and apoptosis inhibitory factor Bcl-2 was significantly down-regulated (Pcells transfected with miR-484 inhibitor; the apoptosis rate of HSC-T6 cells was significantly increased ([32. 81 ±3. 21]% vs [57. 54±6. 76]%, P< 0. 05), and crsmooth muscle actin and collagen type I were both significantly down-regulated (Pcells transfected with miR 484 inhibitor. Conclusion MiR 484 promotes the occurrence and progress of liver fibrosis through inhibiting the apoptosis and promoting the activation of HSC's by targeting Fisl.