Changes of PTEN gene expression in radiation-induced bystander cells / 第二军医大学学报

ABSTRACT

Objective: To study the changes of PTEN gene expression in radiation-induced bystander cells. Methods: The irradiated cells and non-irradiated cells were co-cultured in a ratio of 1:1. The culture medium of irradiated cells was used to culture non-irradiated cells to create two bystander cell models. The expression of PTEN mRNA and protein in the bystander cells, irradiated cells, non-irradiated cells was compared. Results: The protein expression of PTEN in the irradiated cells was negatively associated with the irradiation dose. The down-regulation degree of PTEN protein was related to the different treatments of cells. The most prominent down-regulation was found in the bystander cells cultured with medium of irradiated cells. The down-regulation of PTEN protein in the two kinds of bystander cells was more severe than that in the irradiated cells (P<0.01). Conclusion: PTEN protein expression can be influenced by low dose radiation and the bystander effect of radiation.