Polyethylenimine-mediated BMP-7 gene transfection promotes fracture healing in elderly rats / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 514-518, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-840875
ABSTRACT
Objective:
To observe polyethylenimine-mediated BMP-7 gene transfection in promoting fracture healing in elderly rats.Methods:
Male SD rats (20-month-old) were randomly divided into 3 groups group A received BMP-7 gene therapy mediated by polyehtylenimine, group B was treated with normal saline and group C was treated with BMP-7 plasmid without polyethylenimine. Right femoral shaft fracture model was established in all rats. Animals in group A received transdermal injection of pcDNA3. 1-BMP-7/PET; those in group B and C received the same volume of normal saline and pcDNA3. 1-BMP-7, respectively. X-ray photography, histological observation (H-E staining), immunohistochemical staining of collagen type I, biomechanical and bone mineral density test were employed to assess the healing of fracture 2, 4, and 8 weeks after treatment.Results:
The results of X ray and H-E staining showed no fracture healing in the 3 groups during the 8th week after fracture; however, the growth of coloboma in group A was better than that of the other 2 groups, with partial bone union between the fracture ends. Staining of collagen type I showed deeper, wider staining in group A compared with the other 2 group. Anti-bending intensity and bone mineral density tests showed that the parameters in group A were better than those of the other 2 groups (P<0.05 or 0.01).Conclusion:
Polyethylenimine-mediated hBMP-7 gene transfer can effectively promote fracture healing in elderly rats.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Academic Journal of Second Military Medical University
Année:
2010
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS