Role of JNK pathway and HSP70 in therono-chemotherapy of lung cancer / 第二军医大学学报

ABSTRACT

Objective: To study the effect of thermo-chemotherapy on lung cancer and its possible mechanism. Methods: H446 cells were subjected to different thermo-chemotherapy strategies: 43°C + Paclitaxel (120 μg/L, thermo-chemotherapy group), 43°C+Paclitaxel (120 μg/L)+SP600125 (20 μmol/L, JNK inhibitor) (thermo-chemotherapy+SP600125 group), thermotherapy (43°C) group, and Paclitaxel (120 μg/L) group; untreated cells served as control. MTT assay was used to measure cell proliferation and Western blotting was used to examine the expression of JNK, p-JNK and HSP70 protein. Results: The proliferation rate of cells in the thermo-chemotherapy group was significantly lower than those in the other 3 groups (all P<0.05). The expression of p-JNK was significantly increased in the thermo-chemotherapy group (P<0.05); SF600125 inhibited the expression of p-JNK and the proliferation of cells in the thermo-chemotherapy+ SP600125 group was elevated (P< 0.05). The expression of HSP70 in the thermo-chemotherapy group was lower than that of the thermotherapy group (P<0.05). Conclusion: Thermotherapy can obviously promote the inhibitory effect of Paclitaxel chemotherapy against the growth of lung cancer cell line H446, probably through activating JNK pathway or inhibiting expression of HSP70 protein.