Changes of hepatic function in sepsis mice and protective effects of Leptin on it / 第二军医大学学报

ABSTRACT

Objective: To study the influence of sepsis on hepatic function and corresponding enzymes in mice and to explore the role of Leptin in acute inflammation. Methods: Mice sepsis models were established through cecum ligation and perforation. Mice were divided into sham-operation, sepsis, leptin-protection (peritoneal injection of 0.1 mg/kg Leptin) and indomethacin-protection (peritoneal injection of 2 mg/mg indomethacin) groups. Six and twelve hours after sepsis, leptin levels in liver homogenate were detected by radioimmunoassay; serum alanine aminotransferase (ALT) and 4 enzymes in liver homogenate, myeloperoxidase (MPO), glutathin-S-transferase (GST), xanthine oxidase (XOD) and superoxide dismutase (SOD), all related with synthesis of free radicals, detoxication and purine metabolism, were detected by 96 well spectrophotometry. H-E staining was used to examine the histopathologic changes of liver. Results: Compared with the sham group, sepsis group had an increased serum ALT level (P<0.05) at 12 h after sepsis, but not at 6 h. Serum ALT was significantly lower in Leptin-protection group than in sepsis group 12 h after sepsis (P<0.05). Indomethacin injection had no obvious effect on serum ALT at either 6 h or 12 h after sepsis. Both leptin and indomethacin had no significant effect on hepatic MPO activity, but decreased GST (P<0.01) and SOD (P<0.05) activities and increased XOD (P<0.05) activity. Leptin decreased in sepsis mice but recovered after leptin injection. Indomethacin injection also recovered Leptin level and the level was significantly higher than that of sepsis group at 12 h (P<0.05). Conclusion: Leptin has obviously protective effect on sepsis-induced hepatic injury, the mechanism of which may be related to oxidoreductive reaction, synthesis of oxygen free radicals and detoxication in the metabolic process of hepatic cells.