Protective effect of extractum trametes robiniophila murr on puromycin aminonucleoside-induced podocyte injury in vitro of mice and its mechanism / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of extractum trametes robiniophila murr on the filtration rate, motility and cytoskeleton rearrangement of the podocytes invitro of the puromycin aminonucleoside (PAN) - treated mice, and to clarify the protective effect of extractum trametes robiniophila murr on the podocyte injury and its mechanism. Methods: The podocytes cultured in vitro were randomly divided into control group, model group and test group. The podocytess in model group were treated with 50 mg · L-1 PAN for 24 h; the podocytess in test group were treated with 10 g · L-1 extraction trametes robiniophila murr for 1 h and then treated with 50 mg · L-1 PAN for 24 h. The filtration rate of podocytes to FITC-BSA was measured by two-compartment diffusion system; the scratch repair rate of podocytes was detected by cell scratch test, and the number of podocytes passing through the membrane was measured by Transwell cell migration test. Laser confocal microscope was used to observe the cytoskeleton rearrangement of podocyte cytoskeleton protein F-actin labeled with Invitrogen phalloidin directly. Results: Compared with control group, the FITC-BSA filtration rate of the podocytes in model group was increased significantly (P<0. 01); compared with model group, the FITC-BSA filtration rate of the podocytes in test group was decreased significantly (P<0. 01). Compared with control group, the scratch repair rate of podocytes and the number of transmembrane cells in model group were transmembrane (P<0. 05); compared with model group, the scratch repair rate of podocytes and the number of migration migration cells in test group were decreased significantly (P<0. 05). Compared with control group, the expression level of F-actin in the podocytes in model group was decreased significantly (P<0. 01), the rearrangement rate of F-actin was increased signifiantly (P< 0.01), and the structure of podocyte cytoskeleton was disordered; compared with model group, the expression level of F-actin in the podocytes in test group was increased significantly (P<0. 01), the rearrangement rate of F-actin was decreased significantly (P < 0. 01), and the skeleton rearrangement was alleviated obviously. Conclusion: Extractum trametes robiniophila murr could reduce the filtration rate of podocytes to BSA invitro under the pathological condition, and its possible mechanism is that extractum trametes robiniophila murr reduces the motility of podocytes and improve the rearrangement of podocyte cytoskeleton in vitro.