Therapeutic effects of YiXuanNing Granule on ischemic vertigo of mice and rats and their mechanisms / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the therapeutic effects of YiXuanNing Granule on the ischemic vertigo of the mice and rats, and to clarify its effects on the inflammation damage and oxidative stress in brain tissue of the mice and rats Methods: The vertigo models of mice and rats were established by mechanical vertigo and ligation of bilateral common carotid arteries (CCA), respectively. A total of 60 mice and 60 rats were selected and randomly divided into normal control group, model group, low dose of YiXuanNing Granule group (2.25 g middot; kg-1/1. 50 g middot; kg-1), middle dose of YiXuanNing Granule group (4.50 g middot; kg-1/3. 00 g middot; kg-1), high dose of YiXuanNing Granule group (9.00 g middot; kg-1/6. 00 g middot; kg-1) and positive control group (1.50 g middot; kg-1/1. 00 mg middot; kg -1 flunarizine hydrochloride capsule), and there were 10 animals in each group. The mice and the rats in control group and model group were intragastrically administered with 10 mL middot; kg-1 normal saline, and the mice and the rats in the other groups were given 10 mL middot; kg corresponding drug once a day for 7 d. The escape time of jumping off the platform of the mice and the rats in various groups were determined; the food intake of the mice in various groups were detected; the activities of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) in brain tissue of the rats in various groups were detected by colorimetric method. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in brain tissue of the rats in various groups were detected by ELISA method. Results: Compared with model group, the escape time of jumping off the platform of the mice and the rats in different doses of YiXuanNing Granule groups and positive control group was shortened (P<0. 05 or P<0. 01), and the food intake of the mice in various groups was increased. Compared with model group, the SOD activities in brain tissue of the rats in different doses of YiXuanNing Granule groups were significantly increased (P<0. 05 or P<0. 01); the MDA levels in brain tissue of the rats in different doses of YiXuanNing Granule groups were significantly decreased (P<0. 01); compared with model group, the levels of TNF-α and IL-1β in brain tissue of the rats and the mice in middle and high doses of YiXuanNing Granule groups were significantly decreased (P<0. 05 or P<0. 01). Conclusion: YiXuanNing Granule can inhibit the inflammatory damage in brain tissue of the rats with ischemic vertigo and reduce the oxidative stress, thereby, it can play a therapeutic role in ischemic vertigo.