Analysis on association between single nucleotide polymorphism of selenoprotein S gene and risk of liver cancer / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the distribution of single nucleotide polymorphism (SNP) of selenoprotein S (SelS) gene in the patients with liver cancer and the healthy controls, and to clarify the correlation of the SNP and the risk of liver cancer in the Inner Mongolia Han population. Methods: The distribution of genotypic and allelic frequencies at rs34743744 site was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method among 96 patients with liver cancer and 435 healthy controls, and verified by gene sequencing analysis. The relationship between SNP of SelS gene and the risk of liver cancer was analyzed. Results: There were no differences of the frequencies of CC, CT, and TT genotypes and C and T alleles at rs34713741 site of SelS gene between the patients with liver cancer and the healthy controls(χ=2 679, χ =2. 334, P>0. 05). The differences in the frequencies of CC, CT, and TT genotypes and C and T alleles at rs34743744 site of SelS gene of the drinkers between liver cancer group and healthy control group were significant (χ = 6. 533, χ = 6.447, P<0.05). The relative risk of liver cancer in the drinkers carrying T allele was 2. 297 times of the drinkers carrying genotype CC (OR = 2.297, 95% CI 4.202 - 4.393). Conclusion: The gene-environment interaction of SelS and drinking may associate with liver cancer in the Inner Mongolia Han population.