Effect of LncRNA LOC90024 on viability, migration and invasion of lung cancer cells / 西安交通大学学报(医学版)

ABSTRACT

Objective: To explore the effect of a novel long-noncoding RNA (LncRNA) LOC90024 on cell viability, migration and invasion in A549 cells. Methods: The expression of LOC90024 in lung cancer cells A549 and normal lung cells BEAS-2B was initially screened by Real-time qPCR. The recombinant plasmid of LOC90024 was constructed. The effects of LOC90024 on cell proliferation, migration ability and cell invasion of A549 were detected by Real-time qPCR, MTT, Wound scratch assay and Transwell assay, respectively. Results: Recombinant plasmid with LOC90024 was successfully constructed. Compared with that in blank group, the expression of LOC90024 was significantly increased in overexpression group. MTT results showed that the absorbance value of overexpression LOC90024 group significantly increased in A549 cells as compared with that in blank group. Wound scratch assay showed that the scratch healing ability of overexpression LOC90024 group also significantly increased in A549 cells. Transwell experiment results showed that the number of cells that went through the membrane in overexpression LOC90024 group significantly increased and the A620 value was increased. Conclusion: Overexpression of lncRNA LOC90024 significantly increased cell viability, migration and invasion of A549 cells, indicating that it might function as an oncogene in lung cancer progression and has the prospect of becoming a new target for lung cancer treatment.