Effects of microencapsulated olfactory sheathing cells transplantation on neuropathic pain and the expression of P2X7 receptor in L4.5 spinal cord / 解剖学报

ABSTRACT

Objective To investigate the cflecl of microencapsulated olfactory sheathing cells transplantation on pathological pain induced by peripheral nerve injury and the expression levels of P2X7 receptor in L4., spinal cord. Methods Nash differential adherence method was used to cultivate and expand cells from olfactory bulb tissue of a SD rat. Forty healthy SD rats were randomly divided into control group, model group, OFCs group and microencapsulated OECs group (MC-OECs groups). At the 7th and 14th days after surgery, the mechanical stimulation constraining thresholds of the rats in each group were measured by behavioral method. The experssion levels of P2X7 receptor positive cells percentage and average absorbance by in situ hybridization were observed in L4-J spinal cord. Results At the 7th and 14th days after surgery, compared with the control group, the reflex threshold of mechanical withdrawl of rats in the CCI group significantly decreased(P<0. 05). The percentage and average absorbance of P2X7 receptor positive cells in L4., spinal cord significantly increased(PcO. 05) compared with the CCI group, the reflex threshold of mcchaniral withdrawl of rats in the OF.Cs group was significantly increased (/'cO. 05). The percentage and average absorbance of P2X7 receptor positive cells in L«., spinal cord significantly decreased compared with the OECs group(P<0. 05). The reflex threshold of mechanical withdrawl of rats in the MC-OECs group was higher(/,<0. 05). The percentage and average absorbance of P2X7 receptor positive cells in L4., spinal cord were lower(H<0. 05). Conclusion Microencapsulated olfactory ensheathing cells transplantation can relieve the neuropathic pain belter and reduce the expression levels of P2X7 receptor and repair peripheral nerve injury than OECs transplantate.