RP-HPLC method for the simultaneous determination of daidzein, genistein and formonetin in Solanum Lyratum thunb / 西安交通大学学报·英文版
Academic Journal of Xi'
;
an Jiaotong University;(4): 156-159, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-844717
ABSTRACT
A rapid method for the simultaneous determination of daidzein, genistein and formonetin in Solanum Lyratum Thunb by high performance liquid chromatography (HPLC) was developed. Separation was achieved on a Diamonsil C18 column (250 mm X 4.6 mm, 5 μm) with isocratic elution, using a mobile phase of methanol-tetrahydrofuran-water (44353, v/v). The wavelength was set at 260 nm and column was maintained at 35°C. The linear ranges of daidzein, genistein and formonetin were 1.0-40.0, 0.1-4.0 and 0.1-4.0 μg/mL, respectively. The average recoveries were between 98.4% and 101.3 %. This method could be used for the quality control of Solanum lyratum Thunb due to its simplification, reliability, rapidity and excellent precision.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Academic Journal of Xi'an Jiaotong University
Année:
2010
Type:
Article
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