Relationship between clearing heat and cooling blood and regulating blood redox homeostasis of shikonin based on blood heat syndrome mice model / 中草药

ABSTRACT

Objective: To explore the relationship between clearing heat and cooling blood of shikonin (SK) and regulation of blood redox homeostasis in blood-heat syndrome mice model induced by active yeast. Methods: Mice were randomly divided into control group, model group, SK (10 mg/kg) group, aspirin positive (200 mg/kg) group, L-buthionine sulfoximine (BSO, 600 mg/kg) group, N-acetyl-L-cysteine (NAC, 400 mg/kg) group, BSO combined with SK group, NAC combined with SK group. Mice were sc with 0.2 g/mL active yeast (10 mL/kg) on the neck, mice were ip SK at 0.5 h, ig Asp at 8 h, ip BSO and NAC before the beginning of the experiment (twice a day for one week). The rectal temperature (tR), general activity, metabolism, coagulation, and blood cell counting were determined. The levels of GSH and GSSG were determined by OPA assay. Results: Compared with the control group, tR of mice was significantly increased at 4 h (P < 0.05) and reached (38.07 ± 0.11)℃ at 10 h in model group; Irritability, redder lips, inclined to drinking, reduced carbon powder propelling rate, decreased pellet moisture capacity and prolonged coagulation time were observed (P < 0.05); RBC and inflammatory cells infiltrated were observed in lung tissue sections; The levels of LPO and MDA in serum were significantly increased, the activity of SOD was decreased, GSH/GSSG was decreased (P < 0.05). Compared with model group, tR of mice was significantly reduced and reached (37.51 ± 0.12)℃ at 10 h in SK group; The symptoms were significantly attenuated; The level of LPO and MDA in serum were decreased; The activity of SOD and GSH/GSSG ratio were increased (P < 0.05). BSO significantly reduced the GSH/GSSG ratio (P < 0.05), reduced the effect of SK on blood-heat syndrome mice model. NAC increased the level of GSH in serum and GSH/GSSG ratio (P < 0.05), enhanced the SK treatment effect. Conclusion: SK can attenuate hyperpyrexia, hemorrhage and congestion, oxidative damage in blood-heat syndrome mice model by regulating the blood redox homeostasis, and thereby the effect of clearing heat and cooling blood work.