Determination of 53 pesticide residues in different category of Fritillaria by QuEChERS and GC-MS/MS / 中草药

ABSTRACT

Objective: To establish a method for simultaneous determination of 53 kinds of pesticides residual in different category of Fritillaria by using QuEChERS and gas chromatography tandem mass spectrometry, and applying to 193 batches sample screening. Methods: The forbidden, restricted and commonly used pesticides were selected as detecting indexes according to the result of this investigation. The samples were prepared by QuEChERS, and quantitative analysis was carried out by GC-MS/MS multiple-reaction monitoring (MRM) model. There were three supplemental levels for determining recoveries and RSD. Results: The results showed that all the 53 pesticides had good linearity in certain ranges with the correlation coefficients (R) higher than 0.997 8. The recoveries of more than 86.8% pesticides were ranged from 60% to 140% at three supplemental levels (1×LOD, 2×LOD, and 10×LOD), with the RSDs less than 15%. The LOD ranged of all pesticides were below 0.01 mg/kg. The result of 193 batches sample screening showed that 91 batches sample were detected 14 pesticides, and the detection rate was 47.2%. Conclusion: The detecting indexes is meaningful and the developed method is simple, rapid, sensitive, and reliable for screening multiple pesticide residues in different category of Fritillaria. The result has certain reference value for the cultivation and circulation supervision of different category of Fritillaria.