Correlation study on HPLC fingerprint chromatograms of Astragali Radix decoction pieces-standard decoction-dispensing granules / 中草药

ABSTRACT

Objective: To establish an HPLC fingerprint spectrum of for decoction pieces-, standard decoction- and dispensing granules of Astragali Radix, and evaluate the correlation among them. Methods: HPLC method was used to determine the content of 7-hydroxy- 4'-methoxy isoflavone, calycosin-7-glucoside, calycosin, and ononin in decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. The common pattern of the three fingerprints was established and the correlation was evaluated through the control fingerprint spectrum. Results: The average contents of 7-hydroxy-4'-methoxy isoflavone, calycosin-7-glucoside, calycosin, and ononin in 20 batches of decoction pieces were 0.097, 0.482, 0.142, and 0.237 mg/g, respectively; The average content of these four index components in the standard decoction were 0.116, 0.912, 0.214, and 0.434 mg/g, respectively; The average content of these four index components in the dispensing granules were 0.088,, 0.623,, 0.160, and 0.289 mg/g, respectively. There were six common peaks in all fingerprint spectra of 20 batches of decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. The similarity between dispensing granules and standard decoction was 0.980, indicating that the components difference between them was very small. Conclusion: The study establishes an HPLC fingerprint spectrum, and itwhich reflects the whole picture of multi-component of decoction pieces-, standard decoction- and dispensing granules of Astragali Radix. It provides reference value for the identification and quality control of Astragalus dispensing granules of Astragalus Radix.