Gene expression profiling of light-induced retinal degeneration in phototransduction gene knockout mice
Experimental & Molecular Medicine
;
: 495-504, 2008.
Article
Dans Anglais
| WPRIM
| ID: wpr-84653
ABSTRACT
Exposure to light can induce photoreceptor cell death and exacerbate retinal degeneration. In this study, mice with genetic knockout of several genes, including rhodopsin kinase (Rhok-/-), arrestin (Sag-/-), transducin (Gnat1-/-), c-Fos (c-Fos-/-) and arrestin/transducin (Sag-/-/Gnat1-/-), were examined. We measured the expression levels of thousands of genes in order to investigate their roles in phototransduction signaling in light-induced retinal degeneration using DNA microarray technology and then further explored the gene network using pathway analysis tools. Several cascades of gene components were induced or inhibited as a result of corresponding gene knockout under specific light conditions. Transducin deletion blocked the apoptotic signaling induced by exposure to low light conditions, and it did not require c-Fos/AP-1. Deletion of c-Fos blocked the apoptotic signaling induced by exposure to high intensity light. In the present study, we identified many gene transcripts that are essential for the initiation of light-induced rod degeneration and proposed several important networks that are involved in pro- and anti-apoptotic signaling. We also demonstrated the different cascades of gene components that participate in apoptotic signaling under specific light conditions.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Rétine
/
Dégénérescence de la rétine
/
Transducine
/
Gènes fos
/
Apoptose
/
Souris knockout
/
Séquençage par oligonucléotides en batterie
/
Analyse de profil d'expression de gènes
/
Sous-unités alpha des protéines G
/
Kinase-1 associée au récepteur couplé à une protéine G
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Experimental & Molecular Medicine
Année:
2008
Type:
Article
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