Assembly and sequence analysis of Tetrastigma hemsleyanum chloroplast genome / 中草药

ABSTRACT

Objective: The complete chloroplast genome of medicinal plant Tetrastigma hemsleyanum sequenced by high-throughput technologies was assembled for the sequence analysis to provide evidence for its population genetics and diversity studies. Methods: HiSeq X Ten was used to sequence DNA of T. hemsleyanum, and the chloroplast genome was assembled by NOVOPlasty. Sequence analysis was performed based on gene annotation results. Results: The complete chloroplast genome of T. hemsleyanum was 160 189 bp in length with a GC content of 37.5%. The chloroplast genome exhibited a typical quadripartite structure, including a large single copy region, a pair of inverted repeats, and a small single copy, and the sequence lengths were 88 184 bp, 26 519 bp, and 18 967 bp, respectively. The chloroplast genome harbored 133 genes, including 88 protein-coding genes, eight rRNA genes, and 37 tRNA genes. The ycf1 gene located at the border of IR/SSC was proved to be a pseudogene, with its 3’ end truncated. Conclusion: Sequence assembly and analysis of T. hemsleyanum chloroplast genome provide new insights into future studies on both population genetics and genetic diversity.